PKS/NRPS Domain Search

Choose a domain and query type, then enter your data to identify candidate KS and/or C domains.
Optional Advanced Settings can be used to customize program parameters.

Domain type

KS domains
C domains

Query type

Amino acid sequences
Nucleic acid sequences

Note: Unassembled Illumina sequencing reads from genomes and metagenomes are usually too short to obtain accurate PKS and NRPS domain predictions. Minimum DNA sequence lengths of at least 650 nucleotides are recommended, allowing sequence alignments to cover least 50% of KS or C domains, which are typically about 1250 nucleotides (425 amino acids) long.

Query sequence

Enter or paste sequence(s) in FASTA format, or upload a FASTA file. Please avoid using sequence id numbers containing non-standard characters (e.g. commas, slashes, colons, parentheses, ampersands, etc), as well as ambiguity codes "R" and "Y" for nucleic acid sequence files.

Note: Current size limit for genomic and metagenomic files is 500 MB. Amplicon data queries are currently restricted to < 50,000 sequences. For larger files, please consider our suggestions for file size management before submission. Large nucleotide sequence files may take several minutes to load. We are working on solutions to enable analysis of larger amplicon data sets, but do not yet have a projected availability date.

Upload a file:

Advanced Settings

Sensitivity may be increased by choosing higher e-values and shorter minimum match lengths, but these adjustments may also increase false positives. Conversely, selectivity can be improved with lower e-values and longer minimum match lengths, at the cost of decresed sensitivity.

BLASTP
e-value cutoff

Minimum
align length